Antibody Discovery and Development
- Selection of IRBM Antibody Library and screening strategy based on the specific project requirement
- Recombinant proteins, on cells, in vivo
- Screening by single clone analysis
- automated process for analyzing thousands of individual phage clones
- HTP IgG conversion, production and specificity
- Affinity maturation
- Antibody Characterization and development
- Binding kinetics parameters by SPR or BLI
- Epitope mapping
- Epitope binning
- Binding and affinity on target cells: Cell-Based ELISA, FACS analysis
- Cell-based functional assays: internalization by IF, ADC, ADCC, CDC, cell toxicity, receptor agonism/antagonism
- Tissue specificity by IHC
- In vivo studies: MTD, efficacy in Xenograft models, IHC
