Antibody Phage Display

Phage Display for Antibody Discovery

IRBM’s Phage Display Team is expert at developing and interrogating Phage Display libraries to identify therapeutic antibodies, whichever function you aim to achieve.

Our IRBM Antibody Library (IAL) is a fully human Phage Display library comprised of over 60 billion unique variants, with the diversity and design to deliver therapeutic leads against all types of antigens, including difficult targets. IAL is a single chain fragment variable (scFv) display library possessing extensive diversity in the H-CDR3, with variations carefully selected to mirror the human immune repertoire.

Diagram of a single chain fragment variable displayed on a filamentous phage: the VH and VL domains of an antibody are fused to the phage capsid protein.

In addition to H-CDR3, other H-CDRs and L-CDRs play a crucial role in determining the overall affinity of an antibody. The IRBM Affinity Maturation Library (IAML) includes variations that occur naturally in the H- and L-CDRs of the germlines defining the scFv scaffold of IAL.

Scheme of the IRBM “universal” affinity maturation library: the scFv scaffold with heavy and light chain variable domains joined by a (G4S)3 linker, with diversified positions marked on HCDR1, HCDR2 and the light chain CDRs, while HCDR3 is retained from the primary mAb.

This generates human antibody sequences that represent fully human affinity-matured versions of each variant selected from the naive IAL.

Let’s Talk About Your Antibody Phage Display Project

DISCOVER HOW WE CAN IDENTIFY AND CHARACTERISE YOUR THERAPEUTIC LEAD ANTIBODIES:

ANTIBODY
CHARACTERIZATION→PEPTIDE
PHAGE DISPLAY→

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