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A critical step during drug discovery and development involves producing recombinant proteins in a homogeneous state, devoid of undesired contaminants that can alter their physicochemical properties. This is fundamental to set up in vitro assays that require recombinant proteins as targets during the drug discovery process, or to identify and characterize antibodies specifically directed against a protein of interest. This step is of even greater importance if there is no commercial source for the required protein, or if the commercial sources do not provide the protein at high enough purity or in large enough quantities.

At IRBM, we have a wealth of expertise in recombinant protein production, including antibodies, from a range of expression systems (E.coli, mammalian). Our extensive skill set in designing optimal protein constructs and developing specific purification protocols is best suited to obtaining the protein of interest in a homogeneous and active state. Additionally, we are highly qualified to produce 15N, 13C and 2H isotopically labeled proteins for enabling atomic-resolution structural, dynamic and interaction studies by NMR.

We also have the scientific expertise required to conduct a wide range of quality control studies. These are critical to assess the stability and proper function of the purified protein of interest, particularly if the target of the project is in a complexed format. They also enable us to optimize storage and assay conditions that ensure maximal activity over time.

Protein production can be a crucial step for the advancement of your project. Our team has the necessary expertise to provide your drug discovery and development pipeline with exact amounts of high-purity proteins.

PROTEIN PURIFICATION

  • Affinity chromatography
  • Ion exchange chromatography
  • GST/HIS/MBP/FLAG/STREP II-tagged purification
  • Hydrophobic interaction chromatography (HIC)
  • Size-exclusion chromatography (SEC)
  • Refolding and reconstitution from inclusion bodies

QUALITY CONTROL

  • SDS-PAGE
  • Western Blot
  • ELISA
  • Activity assays
  • SPR
  • NMR
  • DLS
  • MS
FIND OUT MORE ABOUT OUR CROSS FUNCTIONAL DRUG DISCOVERY SERVICES

ASSAY
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ASSAYS

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APPROACHES

STRUCTURAL
BIOLOGY BY NMR

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