Identification of Hit Inhibitors of SARS-CoV-2 NSP13 Helicase through Comprehensive Screening Cascade

About this poster

Targeting NSP13 helicase, an enzyme critical for coronavirus replication and highly conserved across coronaviridae, presents both an opportunity and a challenge in antiviral discovery. This poster outlines a multidisciplinary strategy combining biochemical, biophysical, and fragment-based approaches to identify and characterize NSP13 inhibitors. The established assays, from NMR, ASMS and SPR to ATPase and unwinding assays, now support ongoing hit-to-lead optimization efforts.