Determination of acetyl coenzyme A in human whole blood by ultra-performance liquid chromatography-mass spectrometry

By Speziale, Roberto; Montesano, Camilla; De Leonibus, Maria Lucia; Bonelli, Fabio; Fezzardi, Paola; Beconi, Maria G.; Monteagudo, Edith; Elbaum, Daniel; Orsatti, Laura
From Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences (2018), 1083, 57-62. Language: English, Database: CAPLUS, DOI:10.1016/j.jchromb.2018.02.039

Acetyl CoA is involved in several key metabolic pathways. Its concn. can vary considerably in response to physiol. or pathol. conditions making it a potentially valuable biomarker. However, little information about the measurement and concn. of acetyl CoA in human whole blood is found in the literature. The aim of this study was the development of an accurate method for the detn. of acetyl CoA in human whole blood by LC-MS/MS. The method, involving extn. from whole blood by a rapid protein pptn. procedure was thoroughly validated: limit of quantitation was 3.91 ng mL-1. Accuracy and precision were calcd. at five concns. and were within ± 1 %. The av. endogenous level of acetyl CoA in human whole blood was detd. in 17 healthy individuals to be 220.9 ng mL-1 (ranging from 124.0 to 308.0 ng mL-1). This represents, to the authors’ knowledge, the first report of acetyl CoA levels in human whole blood, and the first practical and reliable method for its detn.