Beghetto E, Gargano N. Whole-Genome Phage Display Libraries: A Powerful Tool for Antigen Discovery. Methods Mol Biol. 2019;2024:181-198. doi: 10.1007/978-1-4939-9597-4_11. PMID: 31364050.

Over the past two decades, phage display technology has become a powerful tool for studying complex biological processes and identifying valuable molecules for various applications. Bacteriophage lambda, a well-known system for cloning and expression, has been utilized to create display libraries of small peptides and protein domains. More recently, researchers have developed large cDNA and whole-genome lambda display libraries from human pathogens to identify novel antigens with biomedical potential. This study highlights the construction of a comprehensive whole-genome library for Streptococcus pneumoniae, a common pathogen, and its application in unraveling the molecular mechanisms of the human B-cell response to bacterial infection and colonization. This approach opens new avenues for understanding host-pathogen interactions and advancing biomedical research.