By Beghetto, Elisa; Gargano, Nicola
From Methods in Molecular Biology (New York, NY, United States) (2019), 2024(Immunoproteomics), 181-198. Language: English, Database: CAPLUS, DOI:10.1007/978-1-4939-9597-4_11
In the last two decades, phage display technol. has been used for investigating complex biol. processes and isolating mols. of practical value in several applications. Bacteriophage lambda, representing a classical cloning and expression system, has also been exploited for generating display libraries of small peptides and protein domains. More recently, large cDNA and whole-genome lambda display libraries of human pathogens have been generated for the discovery of new antigens for biomedical applications. Here, we describe the construction of a whole-genome library of a common pathogen- Streptococcus pneumoniae -and the use of this library for the mol. dissection of the human B-cell response against bacterial infection and colonization.