IRBM has conducted multiple High Throughput Screening (HTS) campaigns and successfully identified chemical matter to support and start drug discovery programs. Two key factors underpin this success:
Factor one
Our robust validated assays that provide reproducible and reliable results
Factor two
Our well curated high-quality collection of compounds
IRBM’s small molecule screening collection is a diverse library of approximately 350,000 compounds with focused subsets. The collection is maintained at -20°C under inert gas to provide the best possible protection for stored compounds and ensure their long-term integrity.
Our accurate and precise transfer of nanoliters of compounds during the screen, eliminates cross contamination and leachates using acoustic dispenser transfer, enabling further assay miniaturization.
The library undergoes regular quality control to monitor compound stability, and the collection remains >90% pure. Additional libraries can be added and hosted.
Our screening platform is a HighRes BioSolutions modular robot – a fully integrated platform with a variety of carts holding different devices. Based on your specific screening campaign, cart swapping provides maximal flexibility to ensure we employ the most appropriate technology.
All operations are tracked by a commercial laboratory information management system to ensure efficient and secure data management and analysis. We currently use a Dotmatics platform for analyses and visualize data using Vortex software.
Together with HTS, we’ll define the most appropriate counter-screening assays to eliminate hits with common nonspecific modes of action. These include:
Redox
Redox Cycling Compound (RCC) assay to eliminate compounds classified as REDOX compounds
DNA binding
DNA intercalator assays to identify compounds that bind DNA
Interference
Classical interference assays to eliminate compounds that are interfering with the platform readout
We can also incorporate wildtype or engineered cell lines into the screening assays and carry out cell viability assessments. In addition, orthogonal assays with biophysical readouts (e.g., SPR, BLI, DSF, MS, NMR) can be applied to further validate identified hits.
Find out more about our high throughput screening technologies:
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